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by Olivier De Bardonneche last modified 27-03-2007 22:40
COMET ASSAY and CELL ARRAY for genotoxicity testing - non-animal alternative for REACH

Commissariat à L'Energie Atomique CEA logo


The CEA has a strategic research role in fundamental pharmaceutical and medical research, diagnostics & therapeutics, biotechnology, and health, environment and food control. Two CEA laboratories will be involved in the COMICS project: The laboratory ‘Biopuces’ (dir. François Chatelain) is in charge of integration of toxicology with the ‘Cell on Chip’ concept. It is developing microsystems dedicated to revolutionary biological analyses with high throughput performances. On a few cm2 of glass or silicon, biochips can manipulate and analyse thousands of different DNA sequences, as many proteins or even individual living cells. The laboratory of nucleic acid lesions (LAN) (dir. Pr Alain Favier) has been active for many years in the identification of DNA base lesions created by chemical and physical agents, and has developed analytical tools (HPLS-MS/MS; comet assay) for the quantitative and qualitative measurement of DNA base lesion in cells, clinical samples and tissues. More recently it has investigated the biological consequences of lesions using modified oligonucleotides (glycosylase substrate characterisation, mutagenic potential).

 

Principal scientific personnel

Béatrice Schaack, PhD (Biopuces): Sylvie Sauvaigo, PhD (LAN).

Recent publications

  1. A ‘DropChip’ cell array for DNA and siRNA transfection combined with drug screening, NanoBiotechnology 1 (2005) p 166-194.
  2. Sauvaigo, S. et al. (2002) Comet assay coupled to repair enzymes for the detection of oxidative damage to DNA induced by low doses of gamma-radiation : use of YOYO-1, low background slides and optimized electrophoresis conditions. Analyt. Bioch. 303, 107-109.
  3. Guerniou, V. et al. (2003) New synthesis of 5-carboxy-2'-deoxyuridine and incorporation into synthetic oligonucleotides. Nucleosides, Nucleotides and Nucleic Acids, 22, 1073-1075.
  4. Sauvaigo, S. et al. (2004) An oligonucleotide microarray for the monitoring of repair enzyme activity toward different DNA base damage. Analyt. Bioch. 333, 182-192.
  5. Hininger, I. et al. (2004) Assessment of DNA damage by comet assay on frozen total blood: method and evaluation in smokers and non-smokers. Mutat. Res. 558, 75-80.

Patents

WO 01/90408, Method for detecting and characterising activity of proteins involved in lesion and DNA repair. Inventor S. Sauvaigo.

WO2004/059004, Method for the quantitative assessment of global and specific DNA repair capacities of at least one biological medium, and the application thereof. Inventor S. Sauvaigo.

comics.vitamib.com

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