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by Olivier De Bardonneche last modified 27-03-2007 22:45

Uppsala University

COMET ASSAY and CELL ARRAY for genotoxicity testing - non-animal alternative for REACH



There is a strong tradition for development of technologies for biological analyses at Uppsala University. The Center for Advanced Molecular Analysis, located at the Rudbeck Laboratory and headed by Professor Ulf Landegren, focuses on developing molecular tools for high-precision analysis of genes and proteins. The center has pioneered a number of important molecular techniques, including padlock probes used directly or amplified via PCR or rolling-circle amplification.

Principle scientific personnel


Associate Professor Mats Nilsson has been developing padlock probe technology since it was first published in 1994; recently combined these probes with rolling-circle amplification to enable single-molecule gene analysis; published 24 scientific papers, 17 review and book chapters; 5 patents/patent applications; heads research group (6 PhD students, two post-docs) with focus on technology development for single-molecule diagnostics; co-founder of Olink AB in Uppsala.

Recent publications

1. Nilsson, M. et al. Padlock probes: Circularizing oligonucleotides for localized DNA detection. Science 265, 2085-2088 (1994). 2. Nilsson, M. et al. Padlock probes reveal single-nucleotide differences, parent of origin and in situ distribution of centromeric sequences in human chromosomes 13 and 21. Nature Genet. 16, 252-255 (1997). 3. Blab, G.A. et al. Sensitive and homogenous detection of single rolling-circle replication products. Anal. Chem. 76, 495-498 (2004). 4. Dahl, F. et al. Circle-to-circle amplification for precise and sensitive DNA analysis. Proc. Natl. Acad. Sci. U S A 101, 4548-4553 (2004). 5. Larsson, C. et al. In situ genotyping individual DNA molecules by target-primed rolling-circle amplification of padlock probes. Nature Meth. 1, 227-232 (2004).

COMET ASSAY and CELL ARRAY for genotoxicity testing - non-animal alternative for REACH
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